Objective: To investigate the clinical value of laboratory diagnostic indexes of alpha-fetoprotein (AFP), Golgi protein 73 (GP73), abnormal plasminogen (DCP), alpha-fetoprotein heterodimer ratio (GP73%), gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), and globulin (Alb) in the diagnosis of primary hepatocellular carcinoma (PHC). Method: Thirty patients with primary liver cancer (liver cancer group) and 41 controls were selected, including 30 patients with hepatitis and 11 patients with healthy physical examination. Compare the serum DCP, GP73, AFP, AFP-L3%, GGT, ALP, Alb levels of the three groups; compare the positive detection rate of each index for primary liver cancer; apply ROC curve to analyze the indexes, and meanwhile analyze and compare the diagnostic value of PHC in individual and We analyzed and compared the diagnostic value of individual and combined tests for PHC. Results: Patients in the hepatocellular carcinoma group had higher DCP, GP73, AFP, AFP-L3%, GGT, and ALP than patients in the liver disease group and the physical examination group, with statistically significant differences; DCP diagnosed the highest positive rate of primary hepatocellular carcinoma; the ROC curves showed that DCP had the largest AUCROC and the highest diagnostic value; the sensitivity of combined test was no difference with the detection of DCP alone. Conclusion: (1) DCP, GP73, AFP, AFP-L3%, GGT, and ALP can play an important role in disease screening for patients with primary hepatocellular carcinoma. (2) DCP is the most valuable single biological indicator for the diagnosis of primary hepatocellular carcinoma, and it has high sensitivity, specificity, and accuracy. (3) Combined assays improved the accuracy of the assays when tested individually but did not differ significantly from the assays for DCP alone.