The informal learning environments play a crucial role in complementing traditional educational settings, catering to the evolving needs of college students for autonomy, creativity, and flexibility. Based on the concept of biophilic design, this paper discusses the application of biophilic theory in informal learning space and improves the quality of learning space by introducing natural elements based on the problems of college students learning pressure and anxiety, combined with the physiological, and psychological and learning behavior characteristics of college students. Drawing on biophilic design theory, this paper analyzes the health benefits of biophilic design, summarizes the elements of biophilic design in informal learning spaces, and tries to put forward design methods and strategies for informal learning spaces in the future. This study aims to understand the relevance of biophilic theory and the design of informal learning spaces, analyze the impact of biophilic spaces on learners’ experiences and outcomes, and provide targeted suggestions for educational practice, to motivate schools, educational institutions, or designers to adopt innovative design concepts to improve learning spaces.