Objective: To explore the effect of the internalization of responsibility ethics in life education of college students, to provide a basis for promoting the internalization of responsibility ethics in life education, to read literature, to compile a questionnaire, to conduct online electronic questionnaire survey on 307 college students, to use statistical software analysis, to interview 24 medical students and analyze the interview results. Results: There were 39 male students (44.3%) and 50 female students (68.5%) who discussed life education with their parents less than female students, the result x2 is 15.504, p is 0.00. Medical students discussed life education with their parents less than non-medical students 62 medical students (54.4%), 127 non-medical students (65.8%), the result x2 is 3.948, p is 0.047. Conclusion: How to implement and promote the internalization of responsibility ethics into college students’ life education needs to be solved, and how to improve the sense of responsibility will be very beneficial to college students’ attention and income on life education.