In this paper, we investigated whether incretin is involved in the process of opioid-induced hyperalgesia (OIH) in rats. The OIH model was prepared by subcutaneous injection of fentanyl citrate in the rat neck. Then, isolating Root’s ganglion (dorsal root ganglion, DRG) cells acutely from the L4-L6 segments of the lumbar spine, we found that the GIP gene in the DRG of the OIH group was significantly up-regulated compared with the control group, while the GLP-1 gene had no significant change compared with the control group by real-time quantitative PCR. Indicating that the GIP gene in incretin may be involved in the peripheral sensitization process of peripheral rats.